EGCG对ZEN所致发育毒性的缓解作用研究开题报告

1. 研究目的与意义

玉米赤霉烯酮是粮食生产、运输、存放中常见的污染物，对人类的健康有多种不良影响。

egcg是绿茶中含量最多的茶多酚，生理活性较强，已被研究证明具有多种对机体有益的作用。

为了研究玉米赤霉烯酮对于机体的毒性是否可以通过egcg缓解，本次选择的论文题目是茶多酚egcg对玉米赤霉烯酮诱导的斑马鱼胚胎发育毒性的保护作用。

2. 研究内容和预期目标

本次毕业论文研究的内容是通过实验收集茶多酚egcg对玉米赤霉烯酮诱导的斑马鱼胚胎发育毒性的保护作用方面的信息。

论文提出的关键问题是：egcg是否可以应用在缓和玉米赤霉烯酮导致的生物发育异常上，以及斑马鱼胚胎发育时玉米赤霉烯酮的毒性如何表现。

论文开头介绍主要的两种实验物质，总结了这两种化合物的历史，展望了它们的发展前景。

3. 国内外研究现状

玉米赤霉烯酮作为一种对人类生活有严重危害的物质，历来是食品化学方面的重要研究对象。

如今，对于玉米赤霉烯酮的化学性质及产生途径等方面已有丰富的研究成果。

斑马鱼拥有其他受试动物没有的优势之处，与果蝇、蛙类、白鼠等试验动物相较，在遗传试验以及发育毒性研究中有着更加优秀的表现。

4. 计划与进度安排

本实验研究以玉米赤霉烯酮和茶多酚egcg为研究对象，以斑马鱼为研究模型，探讨茶多酚egcg对玉米赤霉烯酮诱导的斑马鱼胚胎发育毒性的保护作用研究。

本实验将斑马鱼胚胎暴露于0、1、10、100 ug/l的zen染毒液中120h，发现zen暴露96-120h间诱导斑马鱼出现的心包水肿、脊柱弯曲等畸形率显著升高，并且随着暴露浓度的增加，各畸形的比例增加。

荧光定量pcr结果显示，zen暴露后明显下调了与心脏生长发育有关的基因axin2、sox9b、nkx2.5以及与骨骼生长发育有关的基因bmp-2、bmp-4的表达水平。

5. 参考文献

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